The only two non-pitch differences of pronuncation I know of are the pronunciations of う ,ん ん, and the sokuon. The "u" sound in Kansai-ben is as in European languages and pronounced with rounded lips, not with compressed ones. This also means that lots of devoicing dondoesn't occur, since "u" is now a quite strong sound. Kansai people sometimes even say ~ますうう or ですううう though that is a bit of an exaggeration.
Kansai-ben is much more moraic than Kanto dialect, so ん and っ really do take up one full mora. In Kanto it's more like ん takes up half a mora and the vowel before it is lengthened to 1.5 moras (i.e. かんじ is like "ka-an-ji"). Same with the sokuon (だって is like "da-at-te, this is very obvious in songs). Kansai-ben treats them as full moras, and even pitch accent stress can go upon ん.