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Regarding the use of が instead of は in 「いけのなかにさかな{が・は}たくさんいますか。」

Yes, the particle affects the emphasis. は and が can be used to give emphasis by replacing each other. A sentence where you would normally use は is emphasized by using が, example: 私は学生です。 (I am a ...
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Regarding the use of が instead of は in 「いけのなかにさかな{が・は}たくさんいますか。」

I would go with は. If it were an affirmative declarative sentence (肯定平叙文) I'd use が unless a contrastive meaning is intended: いけのなかにさかながたくさんいます。 There are many fish in the pond. This is a statement/...
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