1
「ぐっ、これは‥
「呪いか!?
「の、呪いなどと‥
「自らを‥ 滅ぼしかねない‥ ものまでを‥使うと‥いうのか‥‥‥ヒトは‥?

It's a monologue from the game. That's the role of の in this sentence「の、呪いなどと‥ ?

3

It is used to represent stuttering speech. 'No-noroi nado to...'.

Without the comma (pause-indicator) it would be more difficult to interpret it as a stutter.

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