I need some additional information on gender differences when speaking Japanese.
の in a question to make sure is used by both male and female people. So the following lines can be naturally said by a male or female speaker.
Assertive sentences which end with の are usually feminine, although there are a few men who use this kind of の. For example,
If native Japanese speakers read or hear these sentences above, most of them would think that a girl or woman is saying the sentences.
の following です or ます in an assertive sentence sounds elegant and very feminine. For example,
However, if the sentence is strongly assertive, の is naturally used by both male and female people like so.
This kind of の can be used in an imperative sentence. For example,
This kind of imperative sentence is also not gender specific.
If の(no) is pronounced longer like のー(nō), it sounds like a male senior’s talking or dialect. This kind of のー is generally written as のう, although う(u) sound is actually not pronounced.
の meaning もの or のもの is used by both male and female people. For example,
According to "A Dictionary of Basic Japanese Grammar", page 322 and 324:
の: a sentence-final particle used by a female speaker or a child to indicate an explanation or emotive emphasis.
The の is used by females or children only in an informal situation. There are times when adult male speakers use の in questions, but they do not use it in declarative sentences.